Study of natural and anthropogenic factors impacting populations of endangered plant species and their sites

Within the framework of the Czech scientific project from the category: Specific research Project - Faculty of Science, University of HK No.2115/2019 "Study of natural and anthropogenic factors impacting populations of endangered plant species and their sites" in 2019-2020, research on Liparis loeselii Liparis loeselii from selected populations in Poland, Slovakia and the Czech Republic continued, using SSR markers and phytosociological and demographic data to assess the variability and genetic structure of this inconspicuous orchid, protected by the Habitats Directive and the Ramsar Convention.

Leaf samples were obtained in 2014-2016, and showed clear genetic distinctiveness of the Czech and Slovak populations from the Polish populations, as well as a lack of gene flow between them. As far as possible, the study included populations with extremely different numbers, as well as representing distinct ecosystems, from initial habitats in sand and gravel pits through low and transitional bogs to carbonate peatlands. The research is part of a broader stream of work, conducted across the EU, on the genetics, demography and effectiveness of existing forms of protection for the species and its wetland habitats.