Prof. Dr. Jan Jaroslaw Rybczynski

Prof. Dr. Jan Jaroslaw Rybczynski

e-mail jan.rybczynski@ob.pan.pl

tel. +48 22 648 38 56 w. 213

  • Honorary Member of the Polish Botanical Society.
  • Awarded the Prof. Wladyslaw Szafer medal for his scientific achievements.
  • For his outstanding contribution to the development of experimental plant biology, he was awarded the Michal Oczapowski Medal by the Faculty of Biological and Agricultural Sciences of the Polish Academy of Sciences.
  • For his contribution to the education of Biotechnologists at the University of Warmia and Mazury, he was honored with the 20th Anniversary Medal of the Faculty of Biology and Biotechnology at UWM in Olsztyn.

Education:

  • He graduated with a degree in biology in 1970 from the Faculty of Biology and Earth Sciences at A. Mickiewicz University.
  • Degree of Doctor of Agricultural Sciences In 1977, Dissertation title: "Regenerative abilities of rye (Secale cereale) embryo fragments in in vitro cultures".
  • Degree of doctor of agricultural sciences  In the field of horticulture-plant biotechnology in 1992, the title of the habilitation thesis " Regeneration of plants in in vitro cultures of some species of the family of  Fabaceae."
  • Title of professor of biological sciences in 1999. the proceedings were conducted by the Council of the Faculty of Biology of Adam Mickiewicz University in Poznan

Course of professional work and major functions (scientific and organizational activities):

  • He began his work in 1970 at the Department of Plant Genetics of the Polish Academy of Sciences, and since 1984 he has been a researcher at the PAN Botanical Garden - CZRB in Powsin. From 1986 to 2012 he was deputy director for scientific affairs except for trips abroad. He headed the Department of Experimental Plant Biology.
  • He was a member of the Biotechnology Committee, the Botany Committee and the Physiology, Genetics and Plant Breeding Committee.
  • Reviewer of MN and S.W. research projects, Fulbright Foundation projects and publications and publications (45 items).

Membership of scientific societies:

  • Polish Botanical Society - from 2004-2010 president, 2001-2004 treasurer, since 1995 member of the Presidium, 1995-2001 Chairman of the Section of in vitro tissue cultures, 1992-2001 vice-chairman of the Section of Cytology and Anatomy.
  • Polish Society for Experimental Plant Biology, one of the founders, President Elect 2003-2005, Polish Genetic Society, Inter. Assos. for Plant Tissue Cullt. and Biotech. now IA for Plant Biotechnology, national correspondent since 1987, American Scientific Society Sigma Xi.
  • Since 1991, Editor-in-Chief of the publication titled Works of the Botanical Garden of the Polish Academy of Sciences.
  • Since 1991 - deputy editor-in-chief of "Bulletin of Botanical Gardens, Museums and Collections, from 2004 to 2006 deputy editor of APP.
  • Since 2007 - Member of the Editorial Committee of APP, currently member of the Editorial Board of BioTechnology, ASBP, ABC-Planta, Herba Polonica.

The main objective of the scientific work is to learn and use the morphogenetic abilities of the plant cell in its manipulation, and the objects of research are monocotyledonous, dicotyledonous and spore plants.

Scope of scientific achievements: Exploiting the morphogenetic abilities of plants at the haploid and diploid levels in manipulating their cells, tissues and organs in vitro, Plant Biotechnology.

  1. Obtaining hybrids of cereal plants (wheat x rye) and grasses (FestuLolio) using in vitro culture of immature hybrid embryos;
  2. To describe the morphogenetic abilities of common rye and selected grasses;
  3. Describing somatic embryogenesis for several species in the rye genus;
  4. To describe the somatic embryogenesis of coarse- and fine-seeded legumes;
  5. To describe the somatic embryogenesis of gentians in agar culture, cell suspension and protoplasts;
  6. Obtaining regenerants by genetic manipulation of gentian somatic cell protoplasts after transformation (indirect and direct ) and somatic hybridization (electrofusion);
  7. Description of the process of androgenesis (haploidization) and transformation of wheat using gene gun;
  8. Cryopreservation of plant material derived from in vitro cultures of seed and spore plants;
  9. To develop in vitro culture of gametophytes and sporophytes of mainly woody ferns in order to study their morphogenetic abilities and physiologically describe cell suspensions of selected species with attention to their metabolome.

Decorations, awards, distinctions etc.:

  • Awarded the Silver Cross of Merit;
  • 2024 - For outstanding contributions to the development of experimental plant biology, honored with the Michal Oczapowski Medal by the Faculty of Biological and Agricultural Sciences of the Polish Academy of Sciences;
  • 2019 - Award of the Division II of Biological and Agricultural Sciences of the Polish Academy of Sciences for the series of works "Discovery and development of a model system of somatic embryogenesis in plants of the clade Monilophyta in vitro". - Member of a 4-person team;
  • 2018 - Distinction of Division II of Biological and Agricultural Sciences of the Polish Academy of Sciences for a series of papers " Morphogenetic potential of tissues, cells and protoplasts and genetic modifications of selected gentian (Gentiana) species". - Chairman of the 4-person team;
  • 2016 - In recognition of his scientific achievements, awarded the Prof. Wladyslaw Szafer Medal by the Polish Botanical Society;
  • 2014 - For his contribution to the education of Biotechnologists at the University of Warmia and Mazury, he was honored with the 20th Anniversary Medal of the Faculty of Biology and Biotechnology at UWM in Olsztyn;
  • 2013 - in recognition of outstanding contributions to the development of botany awarded Honorary Membership of the Polish Botanical Society

Published scientific achievements:

Publication of 224 titles including 117 in scientific journals, books and monographs (original publications - 72, review papers - 26, papers published in Proceedings - 13, monographs and chapters in books - 7, review papers in English - 7) and 107 titles in conference proceedings (international - 49, national - 58).

Alphabetical list of titles of journals in which scientific output has been published:

  • Acta Biologica Cracoviensia - s. Botanica, Acta Horticulture, Acta Physiologiae Plantarum, Acta Socitatis Botanicorum Poloniae;
  • Biodiversity: Research and Conservation, Biological Buletin of Poznań, BioTechnology, Biotechnology, Bulletin of the Institute of Plant Breeding and Acclimatization, Botanical Guidebook, Bulletin of Botanical Gardens;
  • Cells, Cereal Research Communication, Cryoletters;
  • Euphytica, Frontiers in Plant Sciences, Genetica Polonica, Plant Breeding and Seed Science;
  • International Journal of Molecular Sciences, In vitro Cell. Dev. Biol.-Planta, Incompatibility Newsletter, Journal of Applied Genetics;
  • Plant Cell Reports, Plant Cell Tissue, Organ Culture, Plant Science, Proceedings of the Botanical Garden of the Polish Academy of Sciences;
  • Theoretical and Applied Genetics, Zeszyty Naukowe Akademii Rolniczej im. H, Kołłontaja w Krakowie, Zeszyty Problematowe Postępu Nauk Rolniczych'.

Teaching activities:

  • He was the supervisor of 17 master's theses, 5 doctoral dissertations,
  • Professor at the College of Agribusiness. in Lomza, teaches Botany in the basic course and Biotechnology in the master's course.
  • Reviewed 11 doctoral theses, 7 postdoctoral theses, 3 applications for the title of university professor, 3 applications for the title of professor

National forum activity:

For many years he has been active in the organization of scientific life: co-organizer and organizer of 13 cyclic triennial conferences under the activities of the Polish Section of IAPB, two PTB Conventions, and other Polish societies.

Activity in international forums:

  • Establishment and maintenance of the website www: Gentiana.pl
  • Participation in 51 congresses and conferences
  • Implementation of the University of Nebraska Lincoln,USA ''EBSCO Grant,
  • participation in COST Action 871 representative of Poland
  • 51 visits to foreign scientific units,

Scholarship recipient:

  • Fulbright Commission, Agronomy Department, Nebraska University, Lincoln, Nebraska, USA
  • British Council, Botany Department, University Nottingham, Nottingham, UK
  • International Atomic Energy Agency, Agronomy Department, Nebraska University, Lincoln Nebraska, USA
  • U.S. Department of Agriculture, Phytopathology Department, Nebraska University, Lincoln Nebraska, USA
  • Indian National Sciences Academy, Botany Department, Delhi University, India
  • Indian Council of Scientific and Industrial Research, CSIR-National Botanical Research Institute, Lucknow, India,
  • Foundation for Research Development of Republic of South Africa, Kristenbosh Botanical Garden, Cape Town, South Africa,
  • German Academy of Agricultural Sciences, Institute of Agricultural Sciences, Qedlinburg, Germany
  • Hungarian Academy of Nauk, Institute of Plant Genetics, Biological Research Center, Szeged, Hungary
  • Ministry of Agriculture of Taiwan,The Taiwan Forestry Research Institute, Taiwan, China.

Research projects:

Promoter Grants (Manager):

  1. Promoter Grant No. NN304 175540 entitled "Development of in vitro culture and cryopreservation conditions for selected fern species and evaluation of genetic stability of plant material regenerated after cryopreservation". Implementation in the years: 2011-2013.
  2. Promoter grant No. N N302 139035 entitled Use of histochemical and molecular methods to confirm the transgenic nature of in vitro cultures and development of a methodology for genetic transformation of cell suspensions using Agrobacterium tumefaciens" Implementation in years: 2008-2009.

Research Grants (Manager):

  1. The international project COST Action 871 entitled. "Cryopreservation of crop species in Europe". No. 39/N-COST /2007/0 Research project entitled. "Cryopreservation of plant material using In vitro regeneration techniques for the preservation of plant biodiversity". Implementation in years 2007-2010.
  2. Research project No. 3P04C 037 23 entitled "Somatic hybridization of gentians using somatic embryogenesis and transformation". Implementation in years 2002-2006.
  3. Research project No. 5 S301 021 06 entitled Study of morphogenetic abilities of single- and multicellular lupin expolants with special emphasis on Lupinus albus L., L. hispanicus i L. mutabilis. Implementation in years 1994-1996.

Research Grants (Principal Investigator):

  1. Research Project No. UMO-2011/03/B/NZ9/02472 entitled Somatic embryogenesis of ferns - a new phenomenon in experimental plant biology. Cytomorphological-proteomic analysis and cryopreservation. Implementation in the years: 2012-2015.

Book Edition:

  1. Rybczynski J.J., Davey M.R., A. Mikula. 2015. gentianaceae vol. 2: Biotechnology and Application. Springer; Heidelberg, New York, Dordrecht, London. pp.: 1-452
  2. Rybczynski J.J., Davey M.R., A. Mikula. 2014. the Gentianaceae vol.1: Characterization and Ecology. Springer; Heidelberg, New York, Dordrecht, London pp: 1-329
  3. Rybczynski J.J., J.T. Puchalski J 2015 (Eds) Monographs of Botanical Gardens vol. 2: pp: 1-153.
  4. Rybczynski J.J., J.T. Puchalski J 2007.(Eds) Monographs of Botanical Gardens vol. 1: pp:1-151
  5. Rybczynski J.J. 1994.(Ed) Potential applications of in vitro cultures in modern plant breeding. VII All-Poland Conference on In Vitro Cultures. Katowice-Ustroń, Part I (Lectures): pp:1-207, Part II (Posters): pp:1-609.

Chapters in books:

  1. Tomiczak K., Grzyb M., Rybczynski J.J., Mikula A. 2018. Somatic Embryogenesis and Somatic Embryo Cryopreservation of the Tree-Fern Cyathea Delgadii Sternb. In: Jain S., Gupta P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham, pp 291-303. https://doi.org/10.1007/978-3-319-79087-9_23
  2. Mikuła A., Grzyb M., Tomiczak K., Rybczyński J.J. 2018 Experimental and practical application of fern somatic embryogenesis. In: Fernández H. (eds.) Current Advances in Fern Research. Springer, Cham pp 121-137 https://doi.org/10.1007/978-3-319-75103-0_6
  3. Rybczynski J.J., Tomiczak K., Grzyb M., Mikula A. 2018. Morphogenic events in ferns: single and multicellular explants in vitro. In: Fernández H. (eds.) Current Advances in Fern Research. pp. 99-120 Springer, Cham https://doi.org/10.1007/978-3-319-75103-0_5
  4. Rybczynski J.J., Davey M.R., Tomiczak K., Niedziela A., A. Mikula. 2015. systems of plant regeneration in gentian in vitro cultures. In: Gentianaceae vol. 2: Biotechnology and Application (Eds Rybczynski JJ, Davey MR, Mikuła A,), Springer, Heidelberg, New York, Dordrecht, London pp:1-44
  5. Tomiczak K., Mikuła A., J.J. Rybczyński. 2015. protoplast culture and somatic cell hybridization of gentians. In: Gentianaceae vol. 2: Biotechnology and Application (Eds Rybczynski JJ, Davey MR, Mikuła A), Springer, Heidelberg, New York, Dordrecht, London pp:163-185
  6. Mikuła A., Tomiczak K., Domżalska L., J.J. Rybczyński. 2015.Cryopreservation of Gentianaceae: Trends and Applications. In: Gentianaceae vol. 2: Biotechnology and Application (Eds Rybczynski JJ, Davey MR, Mikuła A,), Springer, Heidelberg, New York, Dordrecht, London pp:267-286
  7. Rybczynski J.J., A. Mikula. 2011. tree fern Biotechnology: from spores to sporophytes. Chapter 10 In: Working with Ferns. Issues and Application. (Ed: Ashwani Kumar, Helena Fernandes, Angeles Revilla Bahillo), Springer, pp: 135-147
  8. Mikula A., Makowski D., Walters C., J.J. Rybczynski. 2011. exploration of cryo-methods to preserve fern gametophytes. Chapter 13. in: Working with Ferns. Issues and Application. (Eds: Ashwani Kumar, Helena Fernandes, Angeles Revilla Bahillo), Springer, pp: 173-192
  9. Mikuła A., Rybczyński J.J., Skierski J., Latkowska M.J., A. Fiuk. 2005. somatic embryogenesis of Gentiana genus IV: Characterization of Gentiana cruciata and Gentiana tibetica Embryogenic cell suspensions. Hvoslef-Eide AK, Preis W (eds) In: Liquid culture systems for in vitro plant propagation. pp. 345-358. springer
  10. Rybczynski J.J. 1991. Plant regeneration of selected forage legumes in vitro. Treatises and Monographs 142: 1-64 (in polish).
  11. Zimny J., J.J. Rybczynski, 1986 Somatic embryogenesis of triticale. In: Genetic Manipulation in Plant Breeding. ed: W. Horn et al. 507-510.
  12. Davey M.R., Jothan J.P., Glimur D.M., Ghose T.K., J.J. Rybczynski. 1986. the development of protoplast systems suitable for gene transfer in forage legumes. In: Plant Tissue Culture and its Agricultural Application. (Ed. L.A. Withers), pp: 337-348.

Chapters in post-conference books:

  1. Domżalska L., Mikuła A., Rybczyński J.J. 2016. A view on contemporary trends in the study of the acquisition of plant tolerance in the post-genomic era. Proceeding of the 19th Cold Hardiness Seminar in Poland (Ed: Pukacki P.M. ): 23-30
  2. Domżalska L., Kędracka-Krok S., Rybczyński J.J. 2011. Proteomic changes in Gentiana cruciata L. cell suspension during adaptation to osmotic stress in cryopreservation protocol. Proceeding of the 17th Cold Hardiness Seminar in Poland, Kórnik, Poland May 17-18, 2011; (Ed. P.M. Pukacki): 44-52
  3. Rybczynski J.J., Mikula A. 2009. preparation and storage under conditions of cry Tissues with meristematic and parenchymatic activity. w: Plant Responses to Low Temperature Stress P. Pukacki (ed) IDPAN: 61-67
  4. Rybczyński J.J., Mikuła A. 2007. Biotechnology - an alternative for plant diversity conservation. In: Monographs of Botanical Gardens (Eds: Rybczyński J.J., Puchalski J) vol. 1: 127-132.
  5. Rybczyński J.J., Mikuła A., A. Fiuk. 2005. plant biotechnology in protection of Polish flora. A. Nadolny (ed) in: Biotechnology. Proceedings of the International Review Conference. Editorial Series "Conference Proceedings and Monographs" vol. 5. Polish Academy of Sciences, Vienna, Austria, November 14-18, 2004. 51-60.
  6. Rybczynski J.J., Sawicka-Sienkiewicz E., S. Senktas. 1999. is rooting a problem for lupin propagation through cuttings. In: An ancient crop for the new millennium. Proceeding of the 9th International Conference Klink/Muritz, Germany 29-24 June 1999. pp: 169-171.
  7. Rybczynski J.J., Mikula A., E. Zgagacz. 1999. thidiazuron effect on in vitro response of lupine. In: An ancient crop for the new millennium. Proceeding of the 9th International Conference Klink/Muritz, Germany 29-24 June 1999. pp: 383-387.
  8. Rybczynski J.J. 1999 An assessment of the present status of in vitro culture of Lupins. Towards the 21st Century. Proceeding of the 8th International Lupin Conference Asilomar, California 11-16 May 1996 pp. 222 -235.
  9. Puchalski J.T., Burska A., Rybczynski J.J. 1993 In situ and ex situ conservation of endangered species. Proceedings of Inter. Symp. of Plant Genetic Resources in Europe, Gatersleben, Dec. 6-8. 1993; pp: 202-207.
  10. Rybczynski J.J., J. Zimny, 1985 Screening of cultivar varieties of Secale cereale L. in order to obtain somatic embryogenesis. Eucarpia, Meeting of the Cereals Section of Rye, Svalov, June 11-15, 1985: 33-55.
  11. Zwierzykowski Z., Rybczynski J.J., Slusarkiewicz-Jarzina A., 1985. The use of tissue culture for chromosome doubling of forage grasses. In: Eucarpia Fodder Crops Section. Munchen, Sept.27-20, 1984: 71-77.

Publications:

  1. Rybczynski J., J., Marczak Ł., Stobiecki M., Strugala A., Mikula A. 2022. The metabolite content of the post-culture medium of the tree fern Cyathea delgadii Sternb. cell suspension cultured in the presence of 2,4-D and BAP. International Journal of Molecular Sciences. 23, 11783. https://doi.org/10.3390/ijms231911783
  2. Rybczynski J., J., Podwyszynska M., Tomaszewicz W., Mikula A. 2022. Biotechnology of the tree fern Cyathea smithii Hook.f. (Soft tree fern, Katote). I. Morphogenic potential of shoot apical dome, plant regeneration, nuclear DNA content of regenerants in the presence of the TDZ and NAA. Acta Soc. Bot. Pol. 91, 9133, DOI: 105586/asbp.9133
  3. Rybczynski J., J., Kazmierczak A., Dos Santos Szewczyk K., Tomaszewicz W., Miazga-Karska M., Mikula A. 2022. Biotechnology of the tree fern Cyathea smithii (J.D. Hooker; Soft tree fern, Katote). II. Cell suspension culture: focusing on structure and physiology in the presence of 2,4-D and BAP. Cells 11, 1396. https://doi.org/10.3390/cells11091396
  4. Mikuła A., Gaj M., Grzyb M., Hazubska-Przybył T., Kępczyńska E., Kępczyński J., Rybczyński J., J., Tomiczak K., Wójcik A.,M. 2022. Polish contribution to global research on somatic embryogenesis. Acta Soc. Bot. Pol. 91: 9115, DOI: 105586/asbp.9115.
  5. Mikuła A., Tomaszewicz W., Dziurka M., Kaźmierczak A. Grzyb M., Sobczak M., Zdańkowski P., Rybczyński J. 2021. The origin of the Cyathea delgadii Sternb. Somatic embryo is determined by developmental state of donar tissue and mutual balance of selected metabolites. Cells 10, 1388, 1-28. doi.org/10.3390/cells 100611388
  6. Tomiczak K., Mikuła A., Niedziela A., Wójcik-Lewandowska A., Domżalska L., Rybczyński J.J. 2019 Somatic embryogenesis in the family Gentianaceae and its biotechnological application. Front. Plant Sci. 10: 762. DOI:10.3389/fpls.2019.00762.
  7. Rybczynski J.J., Wojcik A. I. 2019 The effect of L-glutamine on the genetic transformation of embryogenic cell suspensions of gentian species (Gentiana lutea L., Gentiana cruciata L., and Gentiana kurroo Royle) using Agrobacterium tumefaciens. BioTechnology 100 (1) pp: 5-18
  8. Domżalska L., Kędracka-Krok S., Jankowska U., Grzyb M., Sobczak M., Rybczyński J.J., Mikuła A. 2017. Proteomic analysis of stipe explants reveals differentially expressed proteins involved in early direct somatic embryogenesis of the tree fern Cyathea delgadii Sternb. Plant Science 258: 61-76. http://dx.doi.org/10.1016/j.plantsci.2017.01.017
  9. Tomiczak K., Sliwinska E., Rybczynski J.J. 2017. Protoplast fusion in the genus Gentiana: genomic composition and genetic stability of somatic hybrids between Gentiana kurroo Royle and G. cruciata. Plant Cell Tiss Organ Cult (PCTOC) 131:1-14. doi.10.10007/s11240-017-1256-x.
  10. Wojcik A.I., J.J. Rybczynski. 2017. Genetic transformation of gentian Gentiana tibetica (King) leaf explants with Agrobacterium tumefaciens strain C58C1. Acta Physiol _Plant 39:29 (1-16).
  11. Domżalska L., Mikuła A., Rybczyński J.J. 2016. A view on contemporary trends in the study of the acquisition of plant tolerance in the post-genomic era. In: Pukacki P.M. (ed.) Proceedings of the 19th Cold Hardiness Seminar in Poland. 23-30.
  12. Domżalska L., Mikuła A., J.J. Rybczyński. 2016. Protein extraction Ca-alginate encapsulated plant material for comparative proteomic analysis. Protein Extraction and Purification 126: 55-61.
  13. Tomiczak K., Sliwinska E., J. J. Rybczynski. 2016. Comparison of the morphogenic potential of five Gentiana species in leaf mesophyll protoplast culture and ploidy stability of regenerated calli and plants. Plant Cell Tissue Organ Culture 126: 319-331 DOI 10.1007/s11240-016-1000-y.
  14. Makowski D., Tomiczak K., Rybczynski J.J., A. Mikula. 2016. Integration of tissue culture and cryopreservation methods for propagation and conservation of fern Osmunda regalis. Acta Physiol Plant. 38:19.
  15. Makowski D., Rybczynski J.J., A. Mikula. 2015. A simple way to overcome the recalcitrance of the water for Ceratopteris thalictroides (L.) Brongen. to cryopreservation. Acta Soc. Bot. Pol. 84:385-388.
  16. Mikuła A., Pożoga M., Grzyb M., J.J. Rybczyński. 2015. an unique system of somatic embryogenesis in the tree fern Cyathea delgadii Sternb. - The importance of explant type, and physical and chemical factors. Plant Cell Tissue and Organ Culture 123:476-478. DOI 10.1007/s1 1240-015-0850-z.
  17. Wojcik A., J.J. Rybczynski. 2015. Electroporation and morphogenic potential of Gentiana kurroo (Royle) embryogenic cell suspension protoplasts. BioTechnology 96(1): 19-29.
  18. Mikuła A., Tomiczak K., Makowski D., Niedzielski M., J.J. Rybczyński. 2015. the effect of moisture content and temperature on germination and developmental behavior of Osmunda regalis green spores. Acta Physiologiae Plantarum 37:229.
  19. Mikuła A., Pożoga M., Tomiczak K., J.J. Rybczyński. 2015. somatic embryogenesis in ferns: a new experimental system. Plant Cell Reports 34(5):783-94.
  20. Tomiczak K., Mikuła A., Śliwińska E., J.J. Rybczyński. 2015. autotetraploid plant regeneration by indirect somatic embryogenesis from leaf mesophyll protoplasts of diploid Gentiana decumbens L. In vitro Cellular & Developmental Biology; 51:350-359.
  21. Niedziela A., J.J Rybczynski. 2014. Primary results of 2-dimensional electrophoresis for protein studies of Gentiana kurroo Royle somatic embryos derived from long-term embryogenic cell suspension. Acta Soc. Bot. Pol. 83: 247-250.
  22. Mikuła A., Makowski D., Tomiczak K., Rybczyński J.J. 2013. In vitro cultures and cryopreservation in preserving plant diversity - standards for a gene bank. Pol. J. Agronomy 14: 3-17.
  23. Domżalska L., Rybczyński J.J. 2011. Proteomic changes in Gentiana cruciata cell suspension during cryopreservation protocol. In: Cryopreservation of Crop Species in Europe. Proceedings of the Final Meeting of COST Action 871, Angers, France, Feb. 08-11, 2011, pp: 52-55.
  24. Mikuła A., Tomiczak K., Rybczyński J.J. 2011. Cryopreservation enhances embryogenic capacity of Gentiana cruciata (L.) suspension culture and maintains (epi)genetic uniformity of regenerants. Plant Cell Rep. 30: 565-574.
  25. Mikuła A., Tomiczak K., Wójcik A., Rybczyński J.J. 2011. Encapsulation-dehydration method elevates embryogenic abilities of Gentiana kurroo cell suspension and carrying on genetic stability of its regenerants after cryopreservation. Acta Horticult. 908: 143-154.
  26. Fiuk A., Bednarek P., Rybczynski J.J. 2010. Flowcytometry, HPLC-RP, and metAFLP analyses to assess genetic variability in somatic embryo-derived plantlets of Gentiana pannonica Scop. Plant Mol. Biol. Reporter 28: 413-420.
  27. Mikuła A., Jata K., Rybczyński J.J. 2009.Cryopreservation strategies for Cyathea australis (R.BR,) DOMIN. CryoLetters 30: 429-439.
  28. Fiuk A., J.J. Rybczynski. 2008. Factors influencing efficiency of somatic embryogenesis of Gentiana kurroo (Royle) cell suspension. Plant Biotechnol. Rep. 2: 33-39.
  29. Fiuk A., Rybczynski J.J. 2008. Genotype and plant growth regulator-dependent respopnse of somatic embryogenesis from Gentiana spp. leaf explants. In Vitro Cell. Dev. Biol.-Planta 44: 90-99,
  30. Fiuk A., Rybczynski J.J. 2008. morphogenic capability of Gentiana kurroo (Royle) seedlings and leaf explants Acta Physiol Plant 30: 157-166.
  31. Mikuła A., Olasa M., Śliwińska E., Rybczyński J.J. 2008. Cryopreservation by encapsulation of Gentiana spp. cell suspension maintains re-growth, embryogenic competence and DNA content. CryoLetters 29: 409-418.
  32. Goller K., Rybczynski J.J. 2007. gametophyte and sporophyte of tree ferns in vitro Acta Soc. Bot. Pol 76:193-199.
  33. Rybczynski J.J., Borkowska B, Fiuk A, Gawronska H, Sliwinska E, Mikula A 2007 Effect of sucrose concentration on photosynthetic activity of in vitro cultures of Gentiana kurroo (Royle) germlings. Acta Physiol. Plant. 29: 445-453.
  34. Mikuła A., Niedzielski M., Rybczyński J.J. 2006. The use of TTC reduction assay for assessment of Gentiana spp. cell suspension viability after cryopreservation. Acta Physiol. Plant. 28: 315-324.
  35. Mikula A., Rybczynski J.J. 2006. Preliminary studies on cryopreservation and strategies of Cyathea australis gametophyte development in vitro. Botanical Guidebooks 29: 133-142.
  36. Rybczynski J.J., Karolkowska M., Kaczmarek Z., Mikuła A., Fiuk A. 2006. In vitro morphogenic events in culture of Lotus corniculatus' seedling root explants. Acta Soc. Bot. Pol. 3: 191-200.
  37. Rybczyński J.J., Mikuła A. 2006. Engagement of biotechnology in the protection of threatened plant species in Poland. Biodiv. Res. Conserv. 3-4: 361-368.
  38. Mikuła A., Tykarska T., Kuraś M., Rybczyński J.J. 2005 Somatic embryogenesis of Gentiana cruciata (L.): histological and ultrastructural changes in seedling hypocotyl explant. In Vitro Cellular & Developmental Biology - Plant 41: 686-694.
  39. Mikuła A., Fiuk A., Rybczyński J.J. 2005 Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures. Acta Biol. Crac. s Botanica 47: 227-236.
  40. Mikuła A., Tykarska T., Zielińska M., Kuraś M., Rybczyński J.J. 2004. Ultrastructural changes in zygotic embryos of Gentiana punctata (L.) during callus formation and somatic embryogenesis. Acta Biol. Crac. s. Botanica 46: 1-12.
  41. Rybczyński J.J., Mikuła A., Fiuk A.. 2004. endangered species - model plants for experimental botany and biotechnology. Bulletin of Botanical Gardens 13: 59-63.
  42. Mikuła A., Rybczyński J.J., Tykarska T., Kuraś M. 2001. Somatic embryogenesis of Gentiana genus II. Scanning and ultrastructural analysis of early stages of somatic embryogenesis in liquid medium. Biological Bulletin of Poznań (Biological Letters) 38: 47-53.
  43. Święcicki W., Rybczyński J.J., Święcicki W.K. 2000. Domestication and genetics of the yellow lupin (Lupinus luteus L.) and the biotechnological improvement of lupins. J. Appl. Genet. 41 (1) 11-34.
  44. Lingyu Zhang, Rybczynski J.J., Langenberg W.G., Mitra A., French R. 2000. A highly efficient wheat transformation procedure: transformed wheat calli with long term embryogenic potential for plant regeneration. Plant Cell Reports 19: 241-250
  45. Zwierzykowski Z., Rybczynski J.J. 1998. Plant regeneration from immature inflorescence-derived callus of italian ryegrass (Lolium multiflorum Lam.) chromosome number and fertility of regenerants. J. Appl. Gen. 39: 219-224.
  46. Rybczynski J.J. 1997. Plant regeneration in highly embryogenic callus, cell suspension and protoplast cultures of Trifolium fragiferum L. (Leguminose). Plant Cell Tiss. Org. Cult. 51: 159-170.
  47. Zgagacz E.S., Rybczynski J.J. 1996 Different in vitro responses of three species of lupine. J Appl. Gen. 37A: 133-135.
  48. Mikuła A., Wesołowska M., Kapusta J., Skrzypczak L., Rybczyński J.J. 1996 Cytomorphological studies of somatic embryogenesis of Gentiana tibetica (King) and G. cruciata (L.) Acta Soc. Bot. Pol. 65A: 47 - 51.
  49. Rybczynski J.J., Dabrowski L., Badian T. 1995 Changes in morphogenic potential of shoot regeneration from root explant of Lotus corniculatus L. seedling. Acta Physiol. Plant. 17: 211-218.
  50. Goller K., Rybczynski J.J. 1995 In vitro culture used for woody fern Cyanthea australis (R.B.) Domin. Vegetative propagation. Acta Soc. Bot. Pol. 64: 13-17.
  51. Zwierzykowski Z., Rybczynski J.J. 1994. Effectiveness of tissue culture method for chromosome doubling in sterile Lolium - Festuca hybrids. Gen. Pol. 35 A: 73-78.
  52. Rybczynski J.J., W. Kozlowska. 1994. Processes of plant differentiation in vitro and their utilization in genome modification of crop grasses. I. Organogenesis and somatic embryogenesis of explant and callus tissue. Gen. Pol.35 A: 41-48
  53. Badzian T., J.J. Rybczynski. 1994. cytokinin control of shoot regeneration in root segment culture of Lotus corniculatus L. seedling. Acta Physiol. Plant. 16: 61-67.
  54. Rybczynski J.J., Podyma E. 1993 Preliminary studies of plant regeneration via somatic embryogenesis induced on immature cotyledons of white lupin (Lupinus albus) Gen. Pol. 34: 249-257
  55. Sawicka E.J., Rybczynski J.J., Molski B. 1991 Mutation induction in rye (Secale cereale L.). Cereale Research Communication 19: 209-218.
  56. Rybczynski J.J., Simonson R.L. and Baenziger P.S., 1991 Evidence for microspore embryogenesis in wheat anther culture. In Vitro Cell. Dev. Biol. 27P: 168-174.
  57. Rybczynski J.J. 1990 Plant tissue culture of Secale taxa. Euphytica 46: 57-70.
  58. Rybczynski J.J. 1989 Plantlet regeneration and protoplast culture of Browallia speciosa. Plant Cell Reports 8: 383-386.
  59. Rybczynski J.J., H. Gomolinska. 1989.BAP control of the initial bulblet formation of wild lily Lilium martagon L. Acta Horticulture 251: 183-190.
  60. Rybczynski J.J., T. Badian. 1987. High regeneration potential of root segments of Lotus corniculatus seedling on hormone free medium. Plant Science 51: 239-244.
  61. Rybczynski J.J., Zduńczyk W.. 1986. somatic embryogenesis and plantlet regeneration of Secale genus. I. Somatic embryogenesis and organogenesis from cultured immature embryos of five wild species of rye. Theor. Appl. Gen. 73: 267-271.
  62. Rybczynski J.J., Wojciechowska B., Ponitka A. 1986 Somatic embryogenesis and plantlets regeneration from barley x alloplasmatic rye derived callus. Gen. Pol., 27: 16-21
  63. Zwierzykowski Z., Ślusarkiewicz-Jarzina A., J.J. Rybczyński. 1985. Regeneration of plants and chromosome doubling in the tissue culture of Lolium multiflorum Lam. /2n=14/ x Festuca pratensis Hud./2n=14/ hybrids. Gen. Pol. 26: 187-195.
  64. Sodkiewicz W., Rybczynski J.J. 1983 Embryo and endosperm development in caryopsis of hybrids from crosses between tetraploid wheats and their alloplasmic lines with rye. Acta Soc. Bot. Pol. 52: 121-129.
  65. Ponitka A., Rybczynski J.J., A. Slusarkiewicz-Jarzina. 1983. The in vitro application in new genotypes formation of agricultural important plants. Bulletin of the Plant Breeding and Acclimatization, 145: 169-172.
  66. Rybczynski J.J., Stolarz A., A. Śusarkiewicz-Jarzina. 1980. Differentiation of shoot elements from the rachis of Secale cereale L. Acta Soc. Bot. Pol. 49: 161-168.
  67. Rybczynski J.J. 1980 In vitro culture of Secale cereale L. explants. Callus formation and organ differentiation. Acta Soc. Bot. Pol., 49: 155-160.
  68. Sodkiewicz W., Kęsicki E., J.J. Rybczynski. 1980. Successful crossing of diploid wheat and rye. Incompatibility Newsletter 12: 7-10.
  69. Rybczynski J.J. 1979. The influence of cytokinins (BAP, KIN, ZEAT) on the processes of callusing and caulogenesis of the immature embryos of scutellum of di- and tetraploid rye (Secale cereale L.). Gen.Pol. 20: 11-24
  70. Rybczynski J.J. 1978 In vitro culture of the embryo fragments of di- and tetraploid rye (Secale cereale L.). Gen. Pol. 19: 241-251.
  71. Rybczynski J.J. 1978 The effect of the 2,4-D acid on callus formation and rhizogenesis of the immature embryo scutellum of di- and tetraploid rye. Gen. Pol., 19: 467-485.
  72. Rybczynski J.J. 1975 Callus formation and organogenesis of mature cotyledons of Linum usitatissimum L. var. Szokijskij in vitro culture. Gen. Pol. 16: 161-168

Publications in Polish

  1. Rybczynski J.J. 2022. Library and archives of the Polish Botanical Society In: Mostowska A., Rostanski A., Mikula A. (eds.) 2022. Polish Botanical Society on the centenary of its foundation (1922-2022). Polish Botanical Society, Warsaw. 86-95.
  2. Mikula A., Rybczynski J.J. 2008. The versatility of the encapsulation/dehydration technique in protecting diverse plant material. In: Pukacki PM (ed) Papers and reports presented at the XV National Seminar of the Section "Frost Resistance" on May 24-25, 2007 at the Institute of Dendrology of the Polish Academy of Sciences in Kórnik. 47-54.
  3. Rybczynski J.J., A. Mikula 2006. Somatic hybridization of grasses and cereals. In: Sodkiewicz W, Sodkiewicz T, Surma M. (eds.) Remote hybrids of crop plants. 23-33. Institute of Plant Genetics, Polish Academy of Sciences, Poznań.
  4. Rybczynski J.J. 2006.The spread of genetic variation in hybridization within the Gramineae. Biotechnology 4 (75): 136-136
  5. Mikula A., Rybczynski J.J. 2006 Cryopreservation as a tool for long-term storage of cells, tissues and organs derived from in vitro cultures. Biotechnology, 4(75): 145-163
  6. Fiuk A., Tomiczak K., Ładyżyński M., Mikuła A., Rybczyński J.J. 2006. Somatic interspecific hybridization in the genus Gentiana. In: Sodkiewicz W, Sodkiewicz T., Surma M. (eds.) Distant hybrids of crop plants. 191-195. Institute of Plant Genetics, Polish Academy of Sciences, Poznań.
  7. Rybczynski J.J., A. Mikula. 2004. Embryogenic cell suspension a model for studying plant differentiation processes. In Krajewski P, Zwierzykowski Z, Kachlicki P (eds.) Genetics in the improvement of useful plants. Monograph. Institute of Plant Genetics, Polish Academy of Sciences. Poznan, 335-350
  8. Mikula A., J.J. Rybczynski. 2003. Primary explant transformations underlying the acquisition of embryogenic capacity in gentians in vitro culture. Polish Academy of Sciences. Scientific activity - selected issues. 15: 114-117.
  9. Mikula A., J.J. Rybczynski. 2003. Cryopreservation of cell suspensions of selected species of the genus Gentiana. Biotechnology 3(62): 39-51.
  10. Rybczynski J.J., Mikula A 2000. Morphogenetic abilities of cells and their genetic determinants. Biotechnology 2(49): 41-53.
  11. Latkowska M.J., Lukaszewska A.J., Rybczynski J.J., H. Chmiel. 2000. somatic embryo development of common spruce (Picea abies L.) Zeszyty Problematowe Postępów Nauków Rolniczych 473:119-125
  12. Rybczynski J.J., A. Mikula. 1998. use of in vitro cultures in the study of some species of the genus Amaranthus. Plant Breeding and Seed Science, 2: 10-16.
  13. Mikula A., J.J. Rybczynski. 1999. current status of research in biotechnology of plants of the genus Gentiana. Biotechnology 4(47): 1-16.
  14. Mikula A., J.J. Rybczynski. 1998. Scanning and ultrastructural analysis of somatic embryo differentiation from PEM in suspension cultures of Gentiana sp. In: Scientific activities of the Polish Academy of Sciences (selected issues). 63-65.
  15. Mikuła A., Tykarska T., Kuraś M., Rybczyński J.J. 1997 Cytological and ultrastructural analysis of the PEM of Gentiana spp. Conference materials of the 8th All-Poland Conference entitled. "Plant in vitro cultures in basic and applied research". Cracow, 375-378
  16. Mikuła A., Rybczyński J.J. 1997. The use of biotechnology in the behavior of selected species of the genus Gentiana. In: Scientific activities of the Polish Academy of Sciences (selected issues). 44-45
  17. Zgagacz S.E., Rybczynski J.J. 1994. The use of immature embryos of the variable lupin (Lupinus mutabilis) for vegetative multiplication. Proceedings of the Botanical Garden of the Polish Academy of Sciences 5/6: 445-449
  18. Mikula A., Rybczynski J.J. 1994: Somatic embryogenesis in Gentiana spp. Proceedings of the Botanical Garden of the Polish Academy of Sciences 5/6: 425-432
  19. Rybczynski J.J. 1994. regeneration possibilities of root explants. Proceedings of the Botanical Garden of the Polish Academy of Sciences 5/6: 115-124
  20. Kozlowska W., Rybczynski J.J. 1994. Somatic embryogenesis in grasses and cereals. Proceedings of the Botanical Garden of the Polish Academy of Sciences 5/6: 131-138
  21. Kowalczuk A., RybczynskiJ.J.1994. Storage of plant tissues at liquid nitrogen temperature. Proceedings of the Botanical Garden of the Polish Academy of Sciences 5/6: 139-145
  22. Rybczynski J.J., Puchalski J.T. 1993 The use of biotechnology in the establishment of gene banks under ultralow temperature conditions. In: National Conference on Plant and Food Biotechnology. pp:109-113
  23. Rybczynski J.J., Turzynski D. 1992. regenerative abilities of leaf fragments of apple rootstocks and Aronia melanocarpa (Michx) Elliot. Proceedings of the Botanical Garden of the Polish Academy of Sciences 2: 37-44
  24. Rybczynski J.J., Turzynski D., Podyma E., Orszulak J. 1991. Application of in vitro cultures for vegetative propagation of protected species of Polish flora -. Lilium martagon L.. Proceedings of the Botanical Garden 1: 47-54.