Dr. Margaret Grzyb

e-mail malgorzata.grzyb@ob.pan.pl

tel. +48 22 648 38 56 w. 223

Research issues:

  1. Analysis of plant cell differentiation processes in culture in vitro.
  2. Elucidating the determinants of induction and expression of somatic embryogenesis in ferns.
  3. Determine the role of endogenous hormones in the induction of somatic embryogenesis.
  4. Tracking the changes that occur during the formation of somatic embryos from vegetative cells at the physiological and morphological levels (light, electron and confocal microscopy).

Selected publications:

  1. Grzyb M., Mikula A. 2019 Explant type and stress treatment determine the uni- and multicellular origin of somatic embryos in the tree fern Cyathea delgadii Sternb. Plant Cell Tiss Org Cult 136:221-230 https://doi.org/10.1007/s11240-018-1507-5
  2. Tomiczak K., Grzyb M., Rybczynski J.J., Mikula A. (2018) Somatic Embryogenesis and Somatic Embryo Cryopreservation of the Tree-Fern Cyathea Delgadii Sternb. In: Jain S., Gupta P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham, pp 291-303. https://doi.org/10.1007/978-3-319-79087-9_23
  3. Rybczynski J.J., Tomiczak K, Grzyb M, Mikuła A 2018 Morphogenic events in ferns: single and multicellular explants in vitro. In: Fernández H. (eds.) Current Advances in Fern Research. pp. 99-120 Springer, Cham https://doi.org/10.1007/978-3-319-75103-0_5
  4. Mikula A, Grzyb M, Tomiczak K, Rybczynski JJ 2018 Experimental and practical application of fern somatic embryogenesis. In: Fernández H. (eds.) Current Advances in Fern Research. Springer, Cham pp 121-137 https://doi.org/10.1007/978-3-319-75103-0_6
  5. Grzyb M, Kalandyk A, Mikuła A 2018 Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in Cyathea delgadii Sternb. Acta Physiol Plant 40:1. https://doi.org/10.1007/s11738-017-2577-4
  6. Grzyb M., Kalandyk A., Waligórski P., Mikula A. 2017. The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb. Plant Cell Tiss Org Cult 129: 387-397. doi: 10.1007/s11240-017-1185-8 pts; IF 2,002; MNiSW 30.
  7. Domżalska L., Kędracka-Krok S., Jankowska U., Grzyb M., Sobczak M., Rybczyński J.J., Mikuła A. 2017. Proteomic analysis of stipe explants reveals differentially expressed proteins involved in early direct somatic embryogenesis of the tree fern Cyathea delgadii Sternb. Plant Science 258: 61-76. http://dx.doi.org/10.1016/j.plantsci.2017.01.017 pts; IF 3,437; MNiSW 35.
  8. Mikuła A., Pożoga M., Grzyb M., Rybczyński J.J. 2015. An unique system of somatic embryogenesis in the tree fern Cyathea delgadii Sternb. - The importance of explant type, and physical and chemical factors. Plant Cell Tissue and Organ Culture 123: 467-478. doi: 10.1007/s11240-015-0850-z.
  9. Mikuła a., Tomaszewicz W., Dziurka M., Kaźmierczak A. Grzyb M., Sobczak M., Zdańkowski P., Rybczyński J.J. 2021. The origin of the Cyathea delgadii Sternb. Somatic embryo is determined by developmental state of donar tissue and mutual balance of selected metabolites. Cells 10, 1388, 1-28. doi.org/10.3390/cells 100611388

Other forms of activity:

  1. didactics
  • Laboratory exercises for doctoral students of interdisciplinary doctoral studies entitled. "Program for improvement of SGGW didactics in the field of obtaining plant raw materials for energy in the context of the objectives of the Europe 2020 Strategy". POKL.04.03.00-00-042/12-00; SGGW, Faculty of Wood Technology, June-July 2015, Warsaw
  • Supervision of summer internships of SGGW students and interns - Plant Biotechnology Laboratory of PAN OB-CZRB, July-August 2015, Warsaw, Poland
  1. organizational activities

Member of the Organizing Committee of the 14th National Conference on In Vitro Cultures and Plant Biotechnology "Structural, Physiological and Molecular Basis of Plant Differentiation", September 14-17, 2015, Poznań, Poland.